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Literature summary extracted from

  • Ko, M.H.; Puglielli, L.
    Two endoplasmic reticulum (ER)/ER intermediate compartment-based lysine acetyltransferases post-translationally regulate BACE1 levels (2009), J. Biol. Chem., 284, 2482-2492.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.32 membrane transporter for acetyl-CoA and acetyltransferase from cytosol into lumen of the ER/ER Golgi intermediate compartment Homo sapiens
2.3.1.48 membrane transporter for acetyl-CoA and acetyltransferase from cytosol into lumen of the ER/ER Golgi intermediate compartment Homo sapiens

Application

EC Number Application Comment Organism
2.3.1.32 medicine pathogenesis of late-onset Alzheimer disease, post-translational regulation of beta-site amyloid precursor protein-cleaving enzyme 1 (BACE1) levels, a membrane protein that acts as the rate-limiting enzyme in the generation of Alzheimer disease amyloid beta-peptide, acetylation protect the protein from degradation Homo sapiens
2.3.1.48 medicine pathogenesis of late-onset Alzheimer disease, post-translational regulation of beta-site amyloid precursor protein-cleaving enzyme 1 (BACE1) levels, a membrane protein that acts as the rate-limiting enzyme in the generation of Alzheimer disease amyloid beta-peptide, acetylation protect the protein from degradation Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.32 PCR-amplification, stable transfection of CHO cells, with myc-tag, overexpressing enzyme Homo sapiens
2.3.1.48 PCR-amplification, stable transfection of CHO cells, with myc-tag, overexpressing enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.32 siRNA silencing of enzyme gene Homo sapiens
2.3.1.48 siRNA silencing of enzyme gene Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.32 endoplasmic reticulum membrane catalytic site facing the lumen of the endoplasmic reticulum/endoplasmic reticulum Goli intermediate compartment (ER/ERGIC) Homo sapiens 5789
-
2.3.1.32 Golgi membrane catalytic site facing the lumen of the endoplasmic reticulum/endoplasmic reticulum Goli intermediate compartment (ER/ERGIC) Homo sapiens 139
-
2.3.1.48 endoplasmic reticulum membrane catalytic site facing the lumen of the endoplasmic reticulum/endoplasmic reticulum Goli intermediate compartment (ER/ERGIC) Homo sapiens 5789
-
2.3.1.48 Golgi membrane catalytic site facing the lumen of the endoplasmic reticulum/endoplasmic reticulum Goli intermediate compartment (ER/ERGIC) Homo sapiens 139
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.32 20000
-
 SDS-PAGE, rough estimation from figure Homo sapiens
2.3.1.32 28000
-
 SDS-PAGE Homo sapiens
2.3.1.48 20000
-
 SDS-PAGE, rough estimation from figure Homo sapiens
2.3.1.48 28000
-
 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.32 acetyl-CoA + beta-site amyloid precursor protein-cleaving enzyme 1 Homo sapiens
-
 CoA + acetylated beta-site amyloid precursor protein-cleaving enzyme 1
-
 ?
2.3.1.48 acetyl-CoA + beta-site amyloid precursor protein-cleaving enzyme 1 Homo sapiens
-
 CoA + acetylated beta-site amyloid precursor protein-cleaving enzyme 1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.32 Homo sapiens Q9UHF3
-
-
2.3.1.48 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.32 cells are lysed after centrifugation in 100 mM Tris-HCl buffer, pH 7.6, immunoprecipitation, affinity purification with ProFound c-Myc-Tag IP/Co-IP kit, subcellular fractionation of homogenized cells in 10 mM triethanolamine, 10 mM acetic acid, 250 mM sucrose, 1 mM EDTA, and 1 mM dithiothreitol, pH 7.4, centrifuged, membrane pellet resuspended in 5% Nycodenz and layered on top of a Nycodenz solution gradient in 10 mM HEPES, pH 7.4, fractions collected and further concentrated by ultracentrifugation Homo sapiens
2.3.1.48 cells are lysed after centrifugation in 100 mM Tris-HCl buffer, pH 7.6, immunoprecipitation, affinity purification with ProFound c-Myc-Tag IP/Co-IP kit, subcellular fractionation of homogenized cells in 10 mM triethanolamine, 10 mM acetic acid, 250 mM sucrose, 1 mM EDTA, and 1 mM dithiothreitol, pH 7.4, centrifuged, membrane pellet resuspended in 5% Nycodenz and layered on top of a Nycodenz solution gradient in 10 mM HEPES, pH 7.4, fractions collected and further concentrated by ultracentrifugation Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.32 H4 neuroglioma cell
-
 Homo sapiens
-
2.3.1.32 SH-SY5Y cell neuroblastoma Homo sapiens
-
2.3.1.48 H4 neuroglioma cell
-
 Homo sapiens
-
2.3.1.48 SH-SY5Y cell neuroblastoma Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.32 acetyl-CoA + beta-site amyloid precursor protein-cleaving enzyme 1
-
 Homo sapiens CoA + acetylated beta-site amyloid precursor protein-cleaving enzyme 1
-
 ?
2.3.1.32 acetyl-CoA + beta-site amyloid precursor protein-cleaving enzyme 1 50 mM Tris-HCl, pH 8.0, 30°C Homo sapiens CoA + acetylated beta-site amyloid precursor protein-cleaving enzyme 1
-
 ?
2.3.1.32 acetyl-CoA + histone H3 N-terminal tail 50 mM Tris-HCl, pH 8.0, 30°C Homo sapiens CoA + acetylated histone H3 N-terminal tail
-
 ?
2.3.1.48 acetyl-CoA + beta-site amyloid precursor protein-cleaving enzyme 1
-
 Homo sapiens CoA + acetylated beta-site amyloid precursor protein-cleaving enzyme 1
-
 ?
2.3.1.48 acetyl-CoA + beta-site amyloid precursor protein-cleaving enzyme 1 50 mM Tris-HCl, pH 8.0, 30°C Homo sapiens CoA + acetylated beta-site amyloid precursor protein-cleaving enzyme 1
-
 ?
2.3.1.48 acetyl-CoA + histone H3 N-terminal tail 50 mM Tris-HCl, pH 8.0, 30°C Homo sapiens CoA + acetylated histone H3 N-terminal tail
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.32 ATase1
-
 Homo sapiens
2.3.1.32 ATase2
-
 Homo sapiens
2.3.1.32 lysine acetyltransferase
-
 Homo sapiens
2.3.1.48 ATase1
-
 Homo sapiens
2.3.1.48 ATase2
-
 Homo sapiens
2.3.1.48 lysine acetyltransferase
-
 Homo sapiens

Expression

EC Number Organism Comment Expression
2.3.1.32 Homo sapiens transfected cells increase acetyltransferase activity 2fold, and by the treatment with the lipid second messenger ceramide up
2.3.1.32 Homo sapiens transfected cells increase acetyltransferase activity 3fold, and by the treatment with the lipid second messenger ceramide up
2.3.1.48 Homo sapiens transfected cells increase acetyltransferase activity 2fold, and by the treatment with the lipid second messenger ceramide up
2.3.1.48 Homo sapiens transfected cells increase acetyltransferase activity 3fold, and by the treatment with the lipid second messenger ceramide up

General Information

EC Number General Information Comment Organism
2.3.1.32 metabolism beta-site amyloid precursor protein-cleaving enzyme 1 (BACE1) is acetylated in seven lysine residues that face the lumen of the ER and ER Golgi intermediate compartment (ERGIC) Homo sapiens
2.3.1.48 metabolism beta-site amyloid precursor protein-cleaving enzyme 1 (BACE1) is acetylated in seven lysine residues that face the lumen of the ER and ER Golgi intermediate compartment (ERGIC) Homo sapiens